Atlas of Genetics and Cytogenetics in Oncology and Haematology

Home   Genes   Leukemias   Solid Tumors   Cancer-Prone   Deep Insight   Case Reports   Journals  Portal   Teaching   

X Y 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 NA

Polyclonal B Lymphocytosis with Binucleated Lymphocytes (PPBL)

Written2004-06Xavier Troussard, Hossein Mossafa
Laboratoire d'Hématologie, CHU de Caen, 14 000 Caen, France

(Note : for Links provided by Atlas : click)


Atlas_Id 2037

Clinics and Pathology

Phenotype / cell stem origin Unknown. The polyclonal expansion of B-cells fit into the peripheral CD27+IgM+IgD+ B cell population. Cloning and sequencing of VH genes of PPBL IgVH genes showed a mutated profile suggesting like CD27 expression an expansion of memory B cells.
Etiology The etiology of polyclonal B lymphocytosis with binucleated lymphocytes (PPBL) remains unknown. An association with cigarette smoking was initially suggested. However PPBL was observed in non smokers patients. The morphology of binucleated lymphoid B-cells could suggest an association with viral infections, such as Epstein-Barr Virus. Biologic studies are not completely achieved to exclude and/or to confirm definitely the role of EBV in the pathogenesis of PPBL. The presence of characteristic binucleated lymphoid B-cells in asymptomatic family members and the description of familial PPBL cases suggest a genetic predisposition as a more likely possibility.
Epidemiology PPBL was first reported in 1982. We have no epidemiological data on the incidence of PPBL.
Clinics In a large series we reported on forty-three patients (9 males, 34 females: median age: 40 years, range 28-65), the clinical characteristics were splenomegaly in 16%, hepatomegaly in 0.5% and lymph nodes in 11.5% cases. An absolute lymphocytosis > 4 x 109/l is present in 80% of PPBL patients. A persistent, stable and polyclonal increase of IgM levels is usual and most PPBL patients express HLA-DR7. CYTOLOGY_IMAGE lymphocytosisFig1.jpg
Cytology PPBL is identified in all cases by the presence of a variable (1.5 to 9%) number of binucleated peripheral lymphoid cells (Fig 1). The majority of lymphoid cells are large with abundant faintly and basophilic cytoplasm. Characteristic nuclei with a rounded or more commonly irregular form are observed.
Immunologic markers: Both kappa and lambda light-chain are expressed, indicating a polyclonal expansion of the lymphocyte pool. The lymphocytosis is of the B-cell type: the lymphocytes react with CD19, CD20, CD22 and FMC7 antigens.
Morphologic features showing typical binucleated cells
Prognosis After a median follow-up of 5.5 years without treatment, 45 PPBL patients are alive.


Cytogenetics Morphological +i(3q) (Fig 2) is the most common abnormality and is observed in 70% cases, occurring as a single aberration in only a few patients. PCC (Fig 3) is observed in 40% cases and occur rarely as a sole abnormality. Both abnormalities associating +i(3q) and PCC are present in 37% cases.
Using alpha-satellite and telomere chromosome 3 specific probes, +i(3q) is more frequently detected by metaphase FISH studies. (Fig 2).
+i(3q) is rarely described as a recurrent cytogenetic abnormality in patients with hematologic malignancy. Trisomy 3 is reported to be associated with marginal zone B-cell lymphoma. Gain of chromosome 3 or 3q was described in patients with typical clonal b-cell chronic lymphoproliferative disorders, chronic lymphocytic leukemia, prolymphocytic leukemia or Waldenström macroglobulinemia.
A chromosomal instability is present in 67.5% patients These patients present various clonal [ del(6q), +der(8) or +8 or polyploid karyotype] and non clonal chromosomal abnormalities with structural and numerical abnormalities.
This chromosomal instability is variable over time but persist in most cases. In spite of genomic instability, a long follow-up of PPBL patients remains essential and chemotherapy unnecessary.
  Partial karyotype showing +i(3q) -R-banding (left); Detection of I(3q) with telomere chromosome 3q and alpha satellite specific DNA probes (right)
  Premature Chromosome Condensation (PCC)


Chronic B-cell lymphocytosis of the young woman : clinical, phenotypic, and molecular studies.
Delage B, Darveau A, Jacques L, Huot A, Delage JM
Blood,. 1992.
Persistent polyclonal lymphocytosis of B lymphocytes.
Gordon DS, Jones BM, Browning SW, Spira TJ, Lawrence DN
The New England journal of medicine. 1982 ; 307 (4) : 232-236.
PMID 6979709
Persistent polyclonal B-cell lymphocytosis is an expansion of functional IgD(+)CD27(+) memory B cells.
Himmelmann A, Gautschi O, Nawrath M, Bolliger U, Fehr J, Stahel RA
British journal of haematology. 2001 ; 114 (2) : 400-405.
PMID 11529864
Analysis of expressed V(H) genes in persistent polyclonal B cell lymphocytosis reveals absence of selection in CD27+IgM+IgD+ memory B cells.
Loembé MM, Néron S, Delage R, Darveau A
European journal of immunology. 2002 ; 32 (12) : 3678-3688.
PMID 12516560
Chromosomal instability and ATR amplification gene in patients with persistent and polyclonal B-cell lymphocytosis (PPBL).
Mossafa H, Tapia S, Flandrin G, Groupe Français d'Hématologie Cellulaire (GFHC), Troussard X
Leukemia & lymphoma. 2004 ; 45 (7) : 1401-1406.
PMID 15359640
Persistent polyclonal lymphocytosis with binucleated B lymphocytes: a genetic predisposition.
Troussard X, Valensi F, Debert C, Maynadie M, Schillinger F, Bonnet P, Macintyre EA, Flandrin G
British journal of haematology. 1994 ; 88 (2) : 275-280.
PMID 7803270


This paper should be referenced as such :
Troussard, X ; Mossafa, H
Polyclonal B lymphocytosis with binucleated lymphocytes (PPBL)
Atlas Genet Cytogenet Oncol Haematol. 2004;8(3):248-251.
Free journal version : [ pdf ]   [ DOI ]
On line version :

External links

COSMICHisto = - Site = haematopoietic_and_lymphoid_tissue (COSMIC)
arrayMap (UZH-SIB Zurich)[select an item]
REVIEW articlesautomatic search in PubMed
Last year articlesautomatic search in PubMed
All articlesautomatic search in PubMed

© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Mon Aug 12 16:59:34 CEST 2019

Home   Genes   Leukemias   Solid Tumors   Cancer-Prone   Deep Insight   Case Reports   Journals  Portal   Teaching   

For comments and suggestions or contributions, please contact us