CDC20 (cell division cycle 20 homolog (S. cerevisiae))
2008-04-01 Susanta Roychoudhury , Taraswi Banerjee , Somsubhra Nath AffiliationHuman Genetics, Genomics Division, Indian Institute of Chemical Biology, Kolkata-700 032, India
Identity
HGNC
LOCATION
1p34.2
LOCUSID
ALIAS
CDC20A,bA276H19.3,p55CDC
FUSION GENES
DNA/RNA
Description
Start: 43,597,199 bp from pter
End: 43,601,461 bp from pter
Size: 4,262 bases
Orientation: plus strand
13 exons (Entrez), 15 Exons (Ensembl)
End: 43,601,461 bp from pter
Size: 4,262 bases
Orientation: plus strand
13 exons (Entrez), 15 Exons (Ensembl)
Transcription
1697 bp
Pseudogene
No
Proteins
The N-terminal amino acids from 1-153 contains most of the functional domains consisting of the Kinetochore binding domain, Mad2 binding domain and the Anaphase promoting complex (APC) activation domain. Amino acids 129-499 contain the WD-repeat region.
Description
Size: 499 amino acids; 54723 Da. Subunit: Interacts with MAD2L1. The phosphorylated form interacts with APC/C. Developmental stage: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
Expression
It is expressed in all cells, no tissue specificity.
Localisation
Nucleus
Function
CDC20 is a key player in the Spindle Assembly Checkpoint (SAC). When a cell is dividing mitotically, the Metaphase to Anaphase transition is stringently monitored by SAC. After proper alignment of all the sister chromatids to the spindle fibers during metaphase, the Mitotic Checkpoint Complex detaches from CDC20 and free CDC20 protein activates the Anaphase promoting Complex (APC) Activated APC can then degrade Securin which frees the protease Separase. Free Separase can now degrade the Cohesin molecules binding the two sister chromatids together. Upon degradation of Cohesin, the two sister chromatids are free and can migrate to the two spindle poles, thus, initiating Anaphase.
Homology
The C-terminal half is highly conserved from humans to yeast.
Mutations
Germinal
No
Somatic
No
Implicated in
Entity name
Various tumors
Disease
There have been few reports of overexpression of CDC20 in various tumors for eg, greater than 3% of Cdc20 expression was found in bladder cancer, breast cancer, cervical cancer, cholangiocellular carcinoma, AML, CML, colon and rectum carcinoma, esophageal cancer, gastric cancer, gastric cancer (diffuse type), liver cancer, lung cancer (NSCLC), lung cancer (SCLC), osteosarcoma, pancreatic cancer, prostate cancer, renal carcinoma, soft tissue tumor, testicular tumor (Kidoko T, et al., 2007), head and neck cancer (Mondal G, et al., 2007).
Prognosis
No
Cytogenetics
No
Hybrid gene
No
Fusion protein
No
Oncogenesis
Overexpression of CDC20 has been observed in several tumor tissues.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 10377410 | 1999 | Cdc20 associates with the kinase aurora2/Aik. | Farruggio DC et al |
| 11726501 | 2001 | Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. | Fraschini R et al |
| 9628895 | 1998 | Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. | Kallio M et al |
| 17873905 | 2008 | CDC20, a potential cancer therapeutic target, is negatively regulated by p53. | Kidokoro T et al |
| 16497171 | 2006 | A new Mad2-interacting domain of Cdc20 is critical for the function of Mad2-Cdc20 complex in the spindle assembly checkpoint. | Mondal G et al |
| 16777988 | 2007 | Overexpression of Cdc20 leads to impairment of the spindle assembly checkpoint and aneuploidization in oral cancer. | Mondal G et al |
| 12360190 | 2002 | The spindle checkpoint: structural insights into dynamic signalling. | Musacchio A et al |
| 11707408 | 2001 | Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint. | Sironi L et al |
| 15525512 | 2004 | Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. | Tang Z et al |
| 9353311 | 1997 | Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1. | Weinstein J et al |
| 11030144 | 2000 | p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase. | Wu H et al |
| 17612486 | 2007 | Cdc20: a WD40 activator for a cell cycle degradation machine. | Yu H et al |
Other Information
Locus ID:
NCBI: 991
MIM: 603618
HGNC: 1723
Ensembl: ENSG00000117399
Variants:
dbSNP: 991
ClinVar: 991
TCGA: ENSG00000117399
COSMIC: CDC20
RNA/Proteins
| Gene ID | Transcript ID | Uniprot |
|---|---|---|
| ENSG00000117399 | ENST00000310955 | Q12834 |
| ENSG00000117399 | ENST00000372462 | Q12834 |
Expression (GTEx)
Pathways
Protein levels (Protein atlas)
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 38337031 | 2024 | Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling. | 1 |
| 38337031 | 2024 | Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling. | 1 |
| 35319365 | 2023 | Current Progress and Perspectives of CDC20 in Female Reproductive Cancers. | 2 |
| 36764621 | 2023 | CDC20 inhibition alleviates fibrotic response of renal tubular epithelial cells and fibroblasts by regulating nuclear translocation of β-catenin. | 0 |
| 36792756 | 2023 | PRMT6-CDC20 facilitates glioblastoma progression via the degradation of CDKN1B. | 5 |
| 37100900 | 2023 | Alternative CDC20 translational isoforms tune mitotic arrest duration. | 9 |
| 37682144 | 2023 | The potential role of CDC20 in tumorigenesis, cancer progression and therapy: A narrative review. | 3 |
| 38030698 | 2023 | Functional analysis of recurrent CDC20 promoter variants in human melanoma. | 0 |
| 35319365 | 2023 | Current Progress and Perspectives of CDC20 in Female Reproductive Cancers. | 2 |
| 36764621 | 2023 | CDC20 inhibition alleviates fibrotic response of renal tubular epithelial cells and fibroblasts by regulating nuclear translocation of β-catenin. | 0 |
| 36792756 | 2023 | PRMT6-CDC20 facilitates glioblastoma progression via the degradation of CDKN1B. | 5 |
| 37100900 | 2023 | Alternative CDC20 translational isoforms tune mitotic arrest duration. | 9 |
| 37682144 | 2023 | The potential role of CDC20 in tumorigenesis, cancer progression and therapy: A narrative review. | 3 |
| 38030698 | 2023 | Functional analysis of recurrent CDC20 promoter variants in human melanoma. | 0 |
| 34728400 | 2022 | NF2 Gene Participates in Regulation of the Cell Cycle of Meningiomas by Restoring Spindle Assembly Checkpoint Function and Inhibiting the Binding of Cdc20 Protein to Anaphase Promoting Complex/Cyclosome. | 2 |
Citation
Susanta Roychoudhury ; Taraswi Banerjee ; Somsubhra Nath
CDC20 (cell division cycle 20 homolog (S. cerevisiae))
Atlas Genet Cytogenet Oncol Haematol. 2008-04-01
Online version: http://atlasgeneticsoncology.org/gene/40003/cdc20
