| CDH1 (16q22.1) / CDH1 (16q22.1) | CDH1 (16q22.1) / CDKL3 (5q31.1) | CDH1 (16q22.1) / DEXI (16p13.13) |
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CDH1 (16q22.1) / EFNB2 (13q33.3) | CDH1 (16q22.1) / GRTP1 (13q34) | CDH1 (16q22.1) / NPFFR2 (4q13.3) |
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CDH1 (16q22.1) / SIK1 (21q22.3) | CDH1 (16q22.1) / TRHDE (12q21.1) | CDH1 (16q22.1) / VPS50 (7q21.2) |
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CDH1 (16q22.1) / VPS50 (7q21.3) | HSD17B2 (16q23.3) / CDH1 (16q22.1) | IMMT (2p11.2) / CDH1 (16q22.1) |
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MYO15B (17q25.1) / CDH1 (16q22.1) | PAGR1 (16p11.2) / CDH1 (16q22.1) | RPL7A (9q34.2) / CDH1 (16q22.1) |
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TANGO6 (16q22.1) / CDH1 (16q22.1) | TMEM132C (12q24.32) / CDH1 (16q22.1) | VPS50 (7q21.2) / CDH1 (16q22.1) |
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VPS50 (7q21.3) / CDH1 (16q22.1) |
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| Three-dimensional structure of the beta-catenin arm repeat region in complex with the E-cadherin cytoplasmic domain (Huber and Weis, 2001). The arm repeats are formed by three helices, H1 and H2 (both gray) and H3 (blue). Residues 134-161, which include part of the alpha-catenin-binding site and a portion of the first arm repeat, form a single helix in this particular crystal structure (cyan). E-cadherin is divided into five regions of primary structure (1-5) that are indicated in distinct colors (Pokutta S and Weis WI, 2007). |
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Description | The cadherins are a family of calcium-dependent transmembrane linker proteins; the first three that were discovered were named according to their tissue origin (E-cadherin from epithelium, N-cadherin from neural tissue and P-cadherin from placenta). The mature E-cadherin protein consists of three major domains: a large extracellular portion (exons 4-13), which mediates homophilic cellular interactions; and smaller transmembrane (exons 13-14) and cytoplasmic domains (exons 14-16), the latter providing a link to the actin cytoskeleton through an association with various catenins, such as B-catenin. The protein E-cadherin is a calcium-dependent cell-cell adhesion molecule expressed in adherents junctions between epithelial cells. It is a transmembrane glycoprotein with five extracellular domains that mediate intercellular adhesion through homophilic binding. The cytoplasmatic domain is bound to the actin cytoskeleton via intracellular attachment proteins, the catenins. The actin cytoskeleton forms a transcellular network mediating structural integrity, cellular polarity and epithelial morphogenesis. |
Expression | Present tissue specificity for non-neural epithelial tissues and there are high levels in solid tissues. |
Localisation | Cell junction; single-pass type I membrane protein. Anchored to actin microfilaments through association with alpha-catenin, beta-catenin and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. |
Function | One of the most important and ubiquitous types of adhesive interactions required for the maintenance of solid tissues is that mediated by the classic cadherin adhesion molecules. Cadherins are transmembrane Ca2+- dependent homophilic adhesion receptors that are well known to play important roles in cell recognition and cell sorting during development. However, they continue to be expressed at high levels in virtually all solid tissues. There are many members of the classic cadherin family (which is a subset of the larger cadherin superfamily), but E-cadherin in epithelial tissues has been the most studied in the context of stable adhesions. Continued expression and functional activity of E-cadherin are required for cells to remain tightly associated in the epithelium, and in its absence the many other cell adhesion and cell junction proteins expressed in epithelial cells (see below) are not capable of supporting intercellular adhesion. In its capacity to maintain the overall state of adhesion between epithelial cells, E-cadherin is thought to act as an important suppressor of epithelial tumor cell invasiveness and metastasis. |
Homology | Pan troglodytes - CDH1; Canis lupus familiaris - CDH1; Mus musculus - Cdh1; Rattus norvegicus - Cdh1; Gallus gallus - LOC415860; Danio rerio - cdh1 |
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| 57 CDH1 mutations have been found to date. 50 of these are listed in Human Gene Mutation Database. Truncating (27) and splice site (7) mutations are found above the Schema (34/45, 76%), missense mutations below it (11/45, 24%). Two marked with an asterisk have been reported as somatic mutations in sporadic diffuse gastric cancer. No Polymorphisms. No Gross deletions/duplications, complex rearrangements, repeat variations been reported. They spread out all over CDH1 gene (Brooks-Wilson et al., 2004). |
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Germinal | 30 CDH1 germline mutations have been described in hereditary diffuse gastric cancer families. 25 have been inactivating (frameshift, nonsense, and splice-site), the remainders are missense. The mutations are distributed equally throughout the gene. |
Somatic | Somatically acquired mutations in CDH1 were found in about 56% of lobular breast tumors, generally (>90%) in combination with loss of the wild-type allele, while no mutations were found in ductal primary breast carcinomas. Most of these somatic mutations result in premature stop codons as a consequence of insertions, deletions and nonsense mutations. As the majority of these frameshift and nonsense mutations is predicted to generate secreted E-cadherin fragments, the functionality of this major cell-cell adhesion protein is lost. Other cancer-confined E-cadherin mutations also result in crippled proteins. The distinctive invasive growth pattern, which is typical for lobular breast cancers, is fully compatible with this functional inactivation. 472 human tumors and 15 different cancer cell lines derived from 10 different tissues have been screened for CDH1 mutation. So far, frequent somatic mutations (50%) have been identified only in sporadic diffuse gastric cancer (DGC), Lobular Breast Cancer. For sporadic DGC, most somatic mutations are missense (exons 8, 9) or exon skipping. For sporadic Lobular Breast Cancer, most somatic mutations are truncating.472 human tumors and 15 different cancer cell lines derived from 10 different tissues have been screened for CDH1 mutation. So far, frequent somatic mutations (50%) have been identified only in sporadic Diffuse Gastric Cancer, Lobular Breast Cancer. Interestingly, there is a major difference between the mutation types identified in these two carcinoma types. In diffuse gastric carcinomas, the predominant mutations are exon skippings causing in-frame deletions. By contrast, most mutations identified in lobular breast cancer result in premature stop codons. In the case of the diffuse gastric carcinomas, a mutation cluster region is suggested as more than 60% of mutations cause exon skipping of exon 8 and 9. Preliminary in vitro studies using transfected cell lines suggest that tumor-associated E-cadherin mutations reduce cell adhesion, increase cell motility, and change cell morphology possibly by dominant negative mechanisms. On the contrary, the truncating mutations present in lobular breast cancers are obviously scattered over the entire E-cadherin gene. In line with this finding is the observation that the expression of E-cadherin protein is lost in lobular breast cancers, in contrast to the retention of expression of the mutant E-cadherin proteins in diffuse gastric carcinomas. Surprisingly, so far almost no E-cadherin mutations have been found to be located in the highly conserved cytoplasmic domain. In most cases, E-cadherin mutations are found in combination with loss of the wild-type allele. |
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Entity | Non-small cell lung cancer |
Prognosis | Reduced E-cadherin correlates with lymph node metastasis. The rate of vascular invasion was statistically high in cases with the reduced expression of E-cadherin. Reduction of E-cadherin is associated with the degree of differentiation. Bohm et al. found a correlation between differentiation and E-cadherin expression in lung squamous cell carcinoma, and Bongiorno et al. found that well-differentiated lung cancers express E-cadherin, in a preserved fashion, and that poorly differentiated tumors exhibited a reduced or disorganized staining pattern. Sulzer et al. also found that E-cadherin expression significantly correlated with increasing tumor differentiation. In general, undifferentiated or poorly differentiated cancer cells tend to have a strong potential to invade tissues. These results suggest that reduction of E-cadherin correlates with tumor invasion. |
Oncogenesis | Reduced E-cadherin expression weakens cell-to-cell attachment, and tumor cells detach from the primary tumor, invade vessels, and migrate to lymph nodes. Once tumor cells reattach to lymph nodes, E-cadherin is strongly expressed, and lymph nodes are subject to metastases. |
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Entity | Melanoma |
Oncogenesis | The major adhesion mediator between keratinocytes and normal melanocytes is E-cadherin, which disappears during melanoma progression. While normal melanocytes express E-cadherin, this molecule is not found on nevus or melanoma cells. The loss of E-cadherin likely plays a crucial role in tumor progression. Cells that have lost epithelial differentiation, as manifested by the loss of functional E-cadherin, show increased mobility and invasiveness. Keratinocytes can no longer control melanoma cells that have lost E-cadherin. When melanoma cells are forced to express E-cadherin and are cocultured with keratinocytes, they dramatically change: melanomas adhere to keratinocytes, no longer express invasion-related molecules, and lose their invasive capacities |
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Entity | Oesophageal adenocarcinoma |
Prognosis | Reduction in the expression of E-cadherin in patients with OSCC was shown to be strongly associated with postoperative blood borne recurrence, resulting in a poorer prognosis than in those patients with tumours showing normal expression before surgery. This finding suggested that in patients with reduced E-cadherin immunoreactivity, the metastatic potential of the oesophageal cancer cells may be increased. Therefore, the evaluation of E-cadherin immunoreactivity may be useful in predicting haematogenous spread and hence recurrence, thus serving as an aid for planning adjuvant treatment after surgery in patients with OSCC. It has also been reported that E-cadherin might be an independent predictor of micrometastasis in lymph nodes that are classified as N0 by routine histopathological analysis. |
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Differences of E-cadherin expression levels and patterns in primary and metastatic human lung cancer. |
Böhm M, Totzeck B, Birchmeier W, Wieland I |
Clinical & experimental metastasis. 1994 ; 12 (1) : 55-62. |
PMID 8287621 |
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Mutations of the human E-cadherin (CDH1) gene. |
Berx G, Becker KF, Höfler H, van Roy F |
Human mutation. 1998 ; 12 (4) : 226-237. |
PMID 9744472 |
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E-cadherin is inactivated in a majority of invasive human lobular breast cancers by truncation mutations throughout its extracellular domain. |
Berx G, Cleton-Jansen AM, Strumane K, de Leeuw WJ, Nollet F, van Roy F, Cornelisse C |
Oncogene. 1996 ; 13 (9) : 1919-1925. |
PMID 8934538 |
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An efficient and reliable multiplex PCR-SSCP mutation analysis test applied to the human E-cadherin gene. |
Berx G, Nollet F, Strumane K, van Roy F |
Human mutation. 1997 ; 9 (6) : 567-574. |
PMID 9195232 |
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Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1). |
Berx G, Staes K, van Hengel J, Molemans F, Bussemakers MJ, van Bokhoven A, van Roy F |
Genomics. 1995 ; 26 (2) : 281-289. |
PMID 7601454 |
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The E-cadherin/catenin complex: an important gatekeeper in breast cancer tumorigenesis and malignant progression. |
Berx G, Van Roy F |
Breast cancer research : BCR. 2001 ; 3 (5) : 289-293. |
PMID 11597316 |
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E-cadherin expression in primary and metastatic thoracic neoplasms and in Barrett's oesophagus. |
Bongiorno PF, al-Kasspooles M, Lee SW, Rachwal WJ, Moore JH, Whyte RI, Orringer MB, Beer DG |
British journal of cancer. 1995 ; 71 (1) : 166-172. |
PMID 7819034 |
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Germline E-cadherin mutations in hereditary diffuse gastric cancer: assessment of 42 new families and review of genetic screening criteria. |
Brooks-Wilson AR, Kaurah P, Suriano G, Leach S, Senz J, Grehan N, Butterfield YS, Jeyes J, Schinas J, Bacani J, Kelsey M, Ferreira P, MacGillivray B, MacLeod P, Micek M, Ford J, Foulkes W, Australie K, Greenberg C, LaPointe M, Gilpin C, Nikkel S, Gilchrist D, Hughes R, Jackson CE, Monaghan KG, Oliveira MJ, Seruca R, Gallinger S, Caldas C, Huntsman D |
Journal of medical genetics. 2004 ; 41 (7) : 508-517. |
PMID 15235021 |
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E-cadherin and loss of heterozygosity at chromosome 16 in breast carcinogenesis: different genetic pathways in ductal and lobular breast cancer? |
Cleton-Jansen AM |
Breast cancer research : BCR. 2002 ; 4 (1) : 5-8. |
PMID 11879552 |
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Cell adhesion: the molecular basis of tissue architecture and morphogenesis. |
Gumbiner BM |
Cell. 1996 ; 84 (3) : 345-357. |
PMID 8608588 |
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Expression of E-cadherin and beta-catenin in human non-small cell lung cancer and the clinical significance. |
Kase S, Sugio K, Yamazaki K, Okamoto T, Yano T, Sugimachi K |
Clinical cancer research : an official journal of the American Association for Cancer Research. 2000 ; 6 (12) : 4789-4796. |
PMID 11156236 |
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Expression of cell adhesion molecules in oesophageal carcinoma and its prognostic value. |
Nair KS, Naidoo R, Chetty R |
Journal of clinical pathology. 2005 ; 58 (4) : 343-351. |
PMID 15790695 |
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Recent advances in melanoma biology. |
Perlis C, Herlyn M |
The oncologist. 2004 ; 9 (2) : 182-187. |
PMID 15047922 |
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Structure and mechanism of cadherins and catenins in cell-cell contacts. |
Pokutta S, Weis WI |
Annual review of cell and developmental biology. 2007 ; 23 : 237-261. |
PMID 17539752 |
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Expressed cadherin pseudogenes are localized to the critical region of the spinal muscular atrophy gene. |
Selig S, Bruno S, Scharf JM, Wang CH, Vitale E, Gilliam TC, Kunkel LM |
Proceedings of the National Academy of Sciences of the United States of America. 1995 ; 92 (9) : 3702-3706. |
PMID 7731968 |
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Reduced E-cadherin expression is associated with increased lymph node metastasis and unfavorable prognosis in non-small cell lung cancer. |
Sulzer MA, Leers MP, van Noord JA, Bollen EC, Theunissen PH |
American journal of respiratory and critical care medicine. 1998 ; 157 (4 Pt 1) : 1319-1323. |
PMID 9563756 |
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Genetic aetiology of diffuse gastric cancer: so near, yet so far. |
Sweet KM, Lynch HT |
Journal of medical genetics. 2004 ; 41 (7) : 481-483. |
PMID 15235018 |
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