EEF1A2 (eukaryotic translation elongation factor 1 alpha 2)

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EEF1A2 (eukaryotic translation elongation factor 1 alpha 2)

Identity

Other names EEF1AL

EF-1-alpha-2

EF1A

HS1

STN

eEF1A-2

HGNC EEF1A2

Location 20q13.3

Location_base_pair Starts at 61589810 and ends at 61600949 bp from pter ( according to hg18-Mar_2006).

DNA/RNA

Description 8 exons and 7 introns.

Transcription 5 alternatives for transcript splicing.

Protein

Description 50kDa; 463 amino acids contains tRNA binding sites, GTP binding and hydrolysis sites and putative actin binding sites (see figure above).

Expression In mice and humans, eEF1A2 is expressed only in normal tissues of the brain, heart and skeletal muscle. Expression is found in tumours of the breast, lung and ovary.

Localisation Diffusely cytoplasmic and nuclear.

Function This protein is an isoform of the alpha subunit of the elongation factor-1 complex. There are two known isoforms of protein elongation factor eEF1A (eEF1A1 and eEF1A2). eEF1A proteins are GTP-binding proteins that interact with amino-acylated tRNA and recruit them to the ribosome during the elongation phase of protein translation. In addition, eEF1A2 binds to and stimulates the lipid kinase activity of phosphatidylinositol 4-kinase beta, the enzyme that catalyzes the generation of phosphatidylinositol 4-phosphate from phosphatidylinositol. eEF1A2 also activates the Akt serine/threonine kinase and can stimulate actin remodeling and increase the rate of cell invasion and migration in vitro.

Homology EEF1A2 is highly homologous to the EEF1A1. EEF1A2 and EEF1A1 share greater than 90% DNA sequence and amino acid identity. The GTP binding and hydrolysis domains also have homology to the Ras GTPase.

Mutations

Note Inactivation of mouse EEF1A2 leads to immunodeficiency, neuromuscular abnormalities and death by 30 days of age.

Implicated in

Entity Tumorigenesis

Disease Breast, lung and ovarian cancer. EEF1A2 overexpression is found in approximatively 60% of breast, 30% of ovary and 40% of lung tumours.

Prognosis High eEF1A2 protein expression is associated with good prognosis in both breast and ovarian cancer. High eEF1A2 protein expression predicts poor prognosis in lung cancer.

Cytogenetics Fluorescence in situ hybridization (FISH), showed high copy numbers of EEF1A2 in 25% ovarian tumors.

Oncogenesis The eEF1A2 gene is transforming and increases the in-vitro growth rate of mouse and human cells grown in-vitro and also enhances their tumorigenicity in mouse xenograft models.

External links

Nomenclature
HGNC EEF1A2   3192

Entrez_Gene EEF1A2  1917  eukaryotic translation elongation factor 1 alpha 2

Cards
Atlas EEF1A2ID40408ch20q13

GeneCards EEF1A2

Ensembl EEF1A2 [Search_View]   ENSG00000101210 [Gene_View]  EEF1A2 [Vega]

Genatlas EEF1A2
GeneLynx EEF1A2

eGenome EEF1A2

euGene 1917

Genomic and cartography
GoldenPath EEF1A2 - 20q13.3   chr20:61589810-61600949 - 20q13.3   [Description]    (hg18-Mar_2006)

Ensembl EEF1A2 - 20q13.3 [CytoView]
NCBI Mapview

OMIM Disease map [OMIM]

HomoloGene EEF1A2

Gene and transcription
Genbank AB209064 [ ENTREZ ]

Genbank AK307536 [ ENTREZ ]

Genbank BC000432 [ ENTREZ ]

Genbank BC018855 [ ENTREZ ]

Genbank BC110409 [ ENTREZ ]

RefSeq NM_001958 [ SRS ]    NM_001958 [ ENTREZ ]

RefSeq AC_000063 [ SRS ]    AC_000063 [ ENTREZ ]

RefSeq AC_000152 [ SRS ]    AC_000152 [ ENTREZ ]

RefSeq NC_000020 [ SRS ]    NC_000020 [ ENTREZ ]

RefSeq NT_011333 [ SRS ]    NT_011333 [ ENTREZ ]

RefSeq NW_001838671 [ SRS ]    NW_001838671 [ ENTREZ ]

RefSeq NW_927339 [ SRS ]    NW_927339 [ ENTREZ ]

CCDS EEF1A2 CCDS - NCBI

AceView EEF1A2 AceView - NCBI

Unigene Hs.433839 [ SRS ]    Hs.433839 [ NCBI ]     HS433839 [ spliceNest ]

Fast-db 15061 (alternative variants)

Protein : pattern, domain, 3D structure
SwissProt Q05639 [ SRS]    Q05639 [ EXPASY ]     Q05639 [ INTERPRO ]     Q05639 [ UNIPROT ] Q05639 [ VarSplice ]

Prosite PS00301 EFACTOR_GTP [ SRS ]    PS00301 EFACTOR_GTP [ Expasy ]

Interpro IPR000795 ProtSyn_GTP_bd [ SRS ]    IPR000795 ProtSyn_GTP_bd [ EBI ]

Interpro IPR004539 Transl_elong_EF1A_euk/arc [ SRS ]    IPR004539 Transl_elong_EF1A_euk/arc [ EBI ]

Interpro IPR004161 Transl_elong_EFTu/EF1A_2 [ SRS ]    IPR004161 Transl_elong_EFTu/EF1A_2 [ EBI ]

Interpro IPR004160 Transl_elong_EFTu/EF1A_C [ SRS ]    IPR004160 Transl_elong_EFTu/EF1A_C [ EBI ]

CluSTr Q05639

Pfam PF00009 GTP_EFTU [ SRS ]    PF00009 GTP_EFTU [ Sanger ]    pfam00009 [ NCBI-CDD ]

Pfam PF03144 GTP_EFTU_D2 [ SRS ]    PF03144 GTP_EFTU_D2 [ Sanger ]    pfam03144 [ NCBI-CDD ]

Pfam PF03143 GTP_EFTU_D3 [ SRS ]    PF03143 GTP_EFTU_D3 [ Sanger ]    pfam03143 [ NCBI-CDD ]

Blocks Q05639

HPRD 04265

Protein Interaction databases
DIP Q05639
IntAct Q05639
Polymorphism : SNP, mutations, diseases
OMIM 602959    [ map ]

GENECLINICS 602959

SNP EEF1A2 [dbSNP-NCBI]

SNP NM_001958 [SNP-NCI]
SNP EEF1A2 [GeneSNPs - Utah]  EEF1A2] [HGBASE - SRS]

HAPMAP EEF1A2 [HAPMAP]

HGMD EEF1A2

Genetic Association EEF1A2

CDC HuGE EEF1A2

General knowledge
Family Browser EEF1A2 [UCSC Family Browser]

SOURCE NM_001958

SMD Hs.433839

SAGE Hs.433839

GO nucleotide binding [Amigo]  nucleotide binding

GO translation elongation factor activity [Amigo]  translation elongation factor activity

GO GTPase activity [Amigo]  GTPase activity

GO protein binding [Amigo]  protein binding

GO GTP binding [Amigo]  GTP binding

GO nucleus [Amigo]  nucleus

GO cytoplasm [Amigo]  cytoplasm

GO eukaryotic translation elongation factor 1 complex [Amigo]  eukaryotic translation elongation factor 1 complex

GO translational elongation [Amigo]  translational elongation

GO anti-apoptosis [Amigo]  anti-apoptosis

PubGene EEF1A2

TreeFam EEF1A2

CTD 1917 [Comparative ToxicoGenomics Database]

Other databases
Probes
Probe EEF1A2 Related clones (RZPD - Berlin)

PubMed
PubMed 26 Pubmed reference(s) in Entrez

	Nomenclature
HGNC	EEF1A2 3192
Entrez_Gene	EEF1A2 1917 eukaryotic translation elongation factor 1 alpha 2
	Cards
Atlas	EEF1A2ID40408ch20q13
GeneCards	EEF1A2
Ensembl	EEF1A2 [Search_View] ENSG00000101210 [Gene_View] EEF1A2 [Vega]
Genatlas	EEF1A2
GeneLynx	EEF1A2
eGenome	EEF1A2
euGene	1917
	Genomic and cartography
GoldenPath	EEF1A2 - 20q13.3 chr20:61589810-61600949 - 20q13.3 [Description] (hg18-Mar_2006)
Ensembl	EEF1A2 - 20q13.3 [CytoView]
NCBI	Mapview
OMIM	Disease map [OMIM]
HomoloGene	EEF1A2
	Gene and transcription
Genbank	AB209064 [ ENTREZ ]
Genbank	AK307536 [ ENTREZ ]
Genbank	BC000432 [ ENTREZ ]
Genbank	BC018855 [ ENTREZ ]
Genbank	BC110409 [ ENTREZ ]
RefSeq	NM_001958 [ SRS ] NM_001958 [ ENTREZ ]
RefSeq	AC_000063 [ SRS ] AC_000063 [ ENTREZ ]
RefSeq	AC_000152 [ SRS ] AC_000152 [ ENTREZ ]
RefSeq	NC_000020 [ SRS ] NC_000020 [ ENTREZ ]
RefSeq	NT_011333 [ SRS ] NT_011333 [ ENTREZ ]
RefSeq	NW_001838671 [ SRS ] NW_001838671 [ ENTREZ ]
RefSeq	NW_927339 [ SRS ] NW_927339 [ ENTREZ ]
CCDS	EEF1A2 CCDS - NCBI
AceView	EEF1A2 AceView - NCBI
Unigene	Hs.433839 [ SRS ] Hs.433839 [ NCBI ] HS433839 [ spliceNest ]
Fast-db	15061 (alternative variants)
	Protein : pattern, domain, 3D structure
SwissProt	Q05639 [ SRS] Q05639 [ EXPASY ] Q05639 [ INTERPRO ] Q05639 [ UNIPROT ] Q05639 [ VarSplice ]
Prosite	PS00301 EFACTOR_GTP [ SRS ] PS00301 EFACTOR_GTP [ Expasy ]
Interpro	IPR000795 ProtSyn_GTP_bd [ SRS ] IPR000795 ProtSyn_GTP_bd [ EBI ]
Interpro	IPR004539 Transl_elong_EF1A_euk/arc [ SRS ] IPR004539 Transl_elong_EF1A_euk/arc [ EBI ]
Interpro	IPR004161 Transl_elong_EFTu/EF1A_2 [ SRS ] IPR004161 Transl_elong_EFTu/EF1A_2 [ EBI ]
Interpro	IPR004160 Transl_elong_EFTu/EF1A_C [ SRS ] IPR004160 Transl_elong_EFTu/EF1A_C [ EBI ]
CluSTr	Q05639
Pfam	PF00009 GTP_EFTU [ SRS ] PF00009 GTP_EFTU [ Sanger ] pfam00009 [ NCBI-CDD ]
Pfam	PF03144 GTP_EFTU_D2 [ SRS ] PF03144 GTP_EFTU_D2 [ Sanger ] pfam03144 [ NCBI-CDD ]
Pfam	PF03143 GTP_EFTU_D3 [ SRS ] PF03143 GTP_EFTU_D3 [ Sanger ] pfam03143 [ NCBI-CDD ]
Blocks	Q05639
HPRD	04265
	Protein Interaction databases
DIP	Q05639
IntAct	Q05639
	Polymorphism : SNP, mutations, diseases
OMIM	602959 [ map ]
GENECLINICS	602959
SNP	EEF1A2 [dbSNP-NCBI]
SNP	NM_001958 [SNP-NCI]
SNP	EEF1A2 [GeneSNPs - Utah] EEF1A2] [HGBASE - SRS]
HAPMAP	EEF1A2 [HAPMAP]
HGMD	EEF1A2
Genetic Association	EEF1A2
CDC HuGE	EEF1A2
	General knowledge
Family Browser	EEF1A2 [UCSC Family Browser]
SOURCE	NM_001958
SMD	Hs.433839
SAGE	Hs.433839
GO	nucleotide binding [Amigo] nucleotide binding
GO	translation elongation factor activity [Amigo] translation elongation factor activity
GO	GTPase activity [Amigo] GTPase activity
GO	protein binding [Amigo] protein binding
GO	GTP binding [Amigo] GTP binding
GO	nucleus [Amigo] nucleus
GO	cytoplasm [Amigo] cytoplasm
GO	eukaryotic translation elongation factor 1 complex [Amigo] eukaryotic translation elongation factor 1 complex
GO	translational elongation [Amigo] translational elongation
GO	anti-apoptosis [Amigo] anti-apoptosis
PubGene	EEF1A2
TreeFam	EEF1A2
CTD	1917 [Comparative ToxicoGenomics Database]
	Other databases
	Probes
Probe	EEF1A2 Related clones (RZPD - Berlin)
	PubMed
PubMed	26 Pubmed reference(s) in Entrez

Bibliography

Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer.

Anand N, Murthy S, Amann G, Wernick M, Porter LA, Cukier IH, Collins C, Gray JW, Diebold J, Demetrick DJ, Lee JM.

Nat Genet. 2002 Jul;31(3):301-5. Epub 2002 Jun 10.

PMID 12053177

Translation elongation factor eEF1A2 is a potential oncoprotein that is overexpressed in two-thirds of breast tumours.

Tomlinson VA, Newbery HJ, Wray NR, Jackson J, Larionov A, Miller WR, Dixon JM, Abbott CM.

BMC Cancer. 2005 Sep 12;5:113.

PMID 16156888

Expression of eEF1A2 is associated with clear cell histology in ovarian carcinomas: overexpression of the gene is not dependent on modifications at the EEF1A2 locus.

Tomlinson VA, Newbery HJ, Bergmann JH, Boyd J, Scott D, Wray NR, Sellar GC, Gabra H, Graham A, Williams AR, Abbott CM.

Br J Cancer. 2007 May 21;96(10):1613-20. Epub 2007 Apr 17.

PMID 17437010

eEF1A2 activates Akt and stimulates Akt-dependent actin remodeling, invasion and migration.

Amiri A, Noei F, Jeganathan S, Kulkarni G, Pinke DE, Lee JM.

Oncogene. 2007 May 10;26(21):3027-40. Epub 2006 Nov 20.

PMID 17130842

Binding of elongation factor eEF1A2 to phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and phosphatidylinositol 4-phosphate generation.

Jeganathan S, Lee JM.

J Biol Chem. 2007 Jan 5;282(1):372-80. Epub 2006 Nov 6.

PMID 17088255

Expression of protein elongation factor eEF1A2 predicts favorable outcome in breast cancer.

Kulkarni G, Turbin DA, Amiri A, Jeganathan S, Andrade-Navarro MA, Wu TD, Huntsman DG, Lee JM.

Breast Cancer Res Treat. 2007 Mar;102(1):31-41. Epub 2006 Aug 9.

PMID 16897428

The prognostic significance of elongation factor eEF1A2 in ovarian cancer.

Pinke DE, Kalloger SE, Francetic T, Huntsman DG, Lee JM.

Gynecol Oncol. 2008 Mar;108(3):561-8. Epub 2007 Dec 31.

PMID 18164751

REVIEW articles automatic search in PubMed

Last year publications automatic search in PubMed

Search in all EBI NCBI

Contributor(s)

Written 04-2008 Sanna Noubir, Jonathan M Lee

Department of Biochemistry, Microbiology, Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5.

Citation

This paper should be referenced as such :
Noubir S, Lee JM . EEF1A2 (eukaryotic translation elongation factor 1 alpha 2). Atlas Genet Cytogenet Oncol Haematol. April 2008 .
URL : http://AtlasGeneticsOncology.org/Genes/EEF1A2ID40408ch20q13.html

© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Sun Nov 9 19:39:52 2008

Home Genes Leukemias Solid Tumours Cancer-Prone Deep Insight Case Reports Journals Portal Teaching

For comments and suggestions or contributions, please contact us
jlhuret@AtlasGeneticsOncology.org.

Other names	EEF1AL
	EF-1-alpha-2
	EF1A
	HS1
	STN
	eEF1A-2
HGNC	EEF1A2
Location	20q13.3
Location_base_pair	Starts at 61589810 and ends at 61600949 bp from pter ( according to hg18-Mar_2006).

Description	8 exons and 7 introns.
Transcription	5 alternatives for transcript splicing.



Description	50kDa; 463 amino acids contains tRNA binding sites, GTP binding and hydrolysis sites and putative actin binding sites (see figure above).
Expression	In mice and humans, eEF1A2 is expressed only in normal tissues of the brain, heart and skeletal muscle. Expression is found in tumours of the breast, lung and ovary.
Localisation	Diffusely cytoplasmic and nuclear.
Function	This protein is an isoform of the alpha subunit of the elongation factor-1 complex. There are two known isoforms of protein elongation factor eEF1A (eEF1A1 and eEF1A2). eEF1A proteins are GTP-binding proteins that interact with amino-acylated tRNA and recruit them to the ribosome during the elongation phase of protein translation. In addition, eEF1A2 binds to and stimulates the lipid kinase activity of phosphatidylinositol 4-kinase beta, the enzyme that catalyzes the generation of phosphatidylinositol 4-phosphate from phosphatidylinositol. eEF1A2 also activates the Akt serine/threonine kinase and can stimulate actin remodeling and increase the rate of cell invasion and migration in vitro.
Homology	EEF1A2 is highly homologous to the EEF1A1. EEF1A2 and EEF1A1 share greater than 90% DNA sequence and amino acid identity. The GTP binding and hydrolysis domains also have homology to the Ras GTPase.

Entity	Tumorigenesis
Disease	Breast, lung and ovarian cancer. EEF1A2 overexpression is found in approximatively 60% of breast, 30% of ovary and 40% of lung tumours.
Prognosis	High eEF1A2 protein expression is associated with good prognosis in both breast and ovarian cancer. High eEF1A2 protein expression predicts poor prognosis in lung cancer.
Cytogenetics	Fluorescence in situ hybridization (FISH), showed high copy numbers of EEF1A2 in 25% ovarian tumors.
Oncogenesis	The eEF1A2 gene is transforming and increases the in-vitro growth rate of mouse and human cells grown in-vitro and also enhances their tumorigenicity in mouse xenograft models.

REVIEW articles	automatic search in PubMed
Last year publications	automatic search in PubMed

Written	04-2008	Sanna Noubir, Jonathan M Lee
		Department of Biochemistry, Microbiology, Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5.