EIF3F (eukaryotic translation initiation factor 3, subunit F)
2009-07-01 Jiaqi Shi , Mark A Nelson AffiliationDepartment of Surgery, Arizona Cancer Center, University of Arizona, USA (JS); Department of Pathology, Arizona Cancer Center, University of Arizona, USA (JS, MN)
DNA/RNA
Description
10.82kb long.
Transcription
8 exons; transcript length: 3,228 bps; 1073 bp coding sequence.
Pseudogene
Chromosome 2 from 58,332,079 to 58,333,164 bp (AC007250.3, Ensembl).
Proteins
Description
357 amino acids; 37,563.75 Da; Isoelectric point: 5.122; Mov34/MPN/PAD1 domain.
Expression
Ubiquitous.
Localisation
Cytoplasmic. There is also evidence of its existence in the nucleus.
Function
eIF3f is a subunit of eIF3 complex and is highly conserved among species. eIF3f interacts with the caspase-processed isoform of CDK11 (CDK11p46), which appears to be a down-stream effector in apoptotic signaling. eIF3f can be phosphorylated at Ser46 and Thr119 by CDK11p46 during apoptosis. The phosphorylation of eIF3f contributes to translation inhibition and apoptosis. The expression of eIF3f is significantly decreased in pancreatic cancer and melanoma. Loss of the eIF3f allele has been reported in pancreatic cancer and melanoma. Ectopic expression of eIF3f causes rRNA degradation, inhibits translation and cell proliferation, and induces apoptosis in pancreatic cancer and melanoma cells. On the other hand, knockdown of eIF3f prevents apoptosis in pancreatic cancer and melanoma cells. Recent studies also showed decreased cell growth, cell proliferation, colony formation and increased apoptosis in eIF3f-overexpressing NIH3T3 cells.
Homology
eIF3f is not found in S. cerevisiae; However it is found in Schizosaccharomyces pombe (SPBC4C3.07, 35% identity) and in Drosophila Melanogaster (CG9769, 49% identity).
Mutations
Note
No eIF3f mutations have been reported.
Implicated in
Entity name
Pancreatic cancer
Disease
Decreased eIF3f mRNA and protein was found in pancreatic cancer.
Oncogenesis
Decreased eIF3f gene expression may result from loss of eIF3f gene allele and down regulation of transcription. Overexpression of eIF3f in pancreatic cancer and melanoma cells can lead to 28S rRNA degradation, decreased translation and increased apoptosis. On the other hand, knockdown of eIF3f attenuates apoptosis in tumor cells.
Entity name
Melanoma
Disease
Decreased eIF3f mRNA and protein was found in melanoma.
Oncogenesis
See above
Entity name
Muscle atrophy
Disease
eIF3f is a key target of MAFbx, an E3 ubiquitin ligase, during muscle atrophy and has a major role in skeletal muscle hypertrophy. During muscle atrophy, MAFbx targets eIF3f for ubiquitination and degradation. Thus, eIF3f appears to be an attractive therapeutic target.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 18381585 | 2008 | Loss of the eukaryotic initiation factor 3f in melanoma. | Doldan A et al |
| 18658124 | 2008 | All translation elongation factors and the e, f, and h subunits of translation initiation factor 3 are encoded by 5'-terminal oligopyrimidine (TOP) mRNAs. | Iadevaia V et al |
| 19289598 | 2009 | mRNA retrotransposition coupled with 5' inversion as a possible source of new genes. | Kojima KK et al |
| 18354498 | 2008 | The initiation factor eIF3-f is a major target for atrogin1/MAFbx function in skeletal muscle atrophy. | Lagirand-Cantaloube J et al |
| 17581632 | 2007 | Reconstitution reveals the functional core of mammalian eIF3. | Masutani M et al |
| 12446680 | 2003 | The p34cdc2-related cyclin-dependent kinase 11 interacts with the p47 subunit of eukaryotic initiation factor 3 during apoptosis. | Shi J et al |
| 19245811 | 2009 | Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis. | Shi J et al |
| 16532022 | 2006 | Decreased expression of eukaryotic initiation factor 3f deregulates translation and apoptosis in tumor cells. | Shi J et al |
| 19237569 | 2009 | Inhibition of HIV-1 replication by eIF3f. | Valente ST et al |
Other Information
Locus ID:
NCBI: 8665
MIM: 603914
HGNC: 3275
Ensembl: ENSG00000175390
Variants:
dbSNP: 8665
ClinVar: 8665
TCGA: ENSG00000175390
COSMIC: EIF3F
RNA/Proteins
Expression (GTEx)
Pathways
Protein levels (Protein atlas)
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 37544925 | 2023 | eIF3f Mediates SGOC Pathway Reprogramming by Enhancing Deubiquitinating Activity in Colorectal Cancer. | 3 |
| 37544925 | 2023 | eIF3f Mediates SGOC Pathway Reprogramming by Enhancing Deubiquitinating Activity in Colorectal Cancer. | 3 |
| 33736665 | 2021 | EIF3F-related neurodevelopmental disorder: refining the phenotypic and expanding the molecular spectrum. | 3 |
| 33736665 | 2021 | EIF3F-related neurodevelopmental disorder: refining the phenotypic and expanding the molecular spectrum. | 3 |
| 31527668 | 2020 | Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F. | 11 |
| 31527668 | 2020 | Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F. | 11 |
| 30573685 | 2019 | Estrogen receptor α promotes protein synthesis by fine-tuning the expression of the eukaryotic translation initiation factor 3 subunit f (eIF3f). | 14 |
| 30573685 | 2019 | Estrogen receptor α promotes protein synthesis by fine-tuning the expression of the eukaryotic translation initiation factor 3 subunit f (eIF3f). | 14 |
| 26988917 | 2016 | eIF3f reduces tumor growth by directly interrupting clusterin with anti-apoptotic property in cancer cells. | 10 |
| 26988917 | 2016 | eIF3f reduces tumor growth by directly interrupting clusterin with anti-apoptotic property in cancer cells. | 10 |
| 26497985 | 2015 | The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity. | 2 |
| 26497985 | 2015 | The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity. | 2 |
| 24678890 | 2014 | Decreased expression of eukaryotic initiation factor 3f is an adverse prognostic factor for stage I-III gastric cancer. | 5 |
| 24732644 | 2014 | Expression of eukaryotic initiation factor 3f is associated with prognosis in gastric carcinomas. | 7 |
| 24678890 | 2014 | Decreased expression of eukaryotic initiation factor 3f is an adverse prognostic factor for stage I-III gastric cancer. | 5 |
Citation
Jiaqi Shi ; Mark A Nelson
EIF3F (eukaryotic translation initiation factor 3, subunit F)
Atlas Genet Cytogenet Oncol Haematol. 2009-07-01
Online version: http://atlasgeneticsoncology.org/gene/44407/eif3f
