FAM57A (family with sequence similarity 57, member A)

2010-08-01   Zhiao Chen , Xianghuo He 

State Key Laboratory of Oncogenes, Related Genes, Shanghai Cancer Institute, Shanghai, China

Identity

HGNC
LOCATION
17p13.3
LOCUSID
ALIAS
CT120,FAM57A
FUSION GENES

DNA/RNA

Description

Gene size: 2145 bp in length, ORF 774 bp.
Full-length cDNA of CT120/FAM57A contains 2145 base pairs and encodes a protein with 257 amino acids.

Transcription

The CT120 contains two isoforms in human: one isoform identified was termed CT120A; another isoform (AAH26023.1) was named CT120B, which consists of four exons and encodes a protein with 225 amino acids (the fourth exon in CT120A is spliced).

Proteins

Atlas Image

Description

- CT120: 257 aa; 29 kDa.
- CT120B: 225 aa; 25 kDa.

Expression

CT120 is universally expressed in different human normal tissues and in various human tumor cell lines.

Localisation

CT120 is a novel plasma membrane-associated gene.

Function

CT120 may assume very essential physiological functions involving in amino acid transport and glutathione metabolism through interaction with SLC3A2 and GGTL3B.

Homology

Homology comparison revealed that CT120 is highly conserved during biological evolution.

Implicated in

Entity name
Lung cancer
Prognosis
CT120A protein was a potential molecular target for treatment of lung cancers. CT120A was overexpressed in 15 cases of the 16 primary lung cancer specimens. Knockdown of CT120A by small hairpin RNA in the human lung adenocarcinoma cell line SPC-A-1 cells resulted in a reduced cell growth rate in vitro and decrease of the capacity for anchorage-independent growth and tumorigenicity in nude mice.
The suppression of CT120A expression also sensitized cells to ultraviolet-induced apoptosis. Atlas cDNA expression array revealed that the expressions of several apoptosis- and growth-associated genes were altered underlying the molecular mechanisms of these cell biological behaviors.
Oncogenesis
CT120 ectopic expression could promote cell proliferation activity of NIH3T3 cells, and two major signaling pathways involved in cell proliferation, cell survival and anti-apoptosis were overexpressed and activated in response to CT120: one is the Raf/MEK/Erk signal cascades and the other is the PI3K/Akt signal cascades, suggesting that CT120 might contribute, at least in part, to the constitutively activation of Erk and Akt in human lung cancer cells.
In addition, some tumor metastasis associated genes cathepsin B, cathepsin D, cathepsin L, MMP-2/TIMP-2 were also upregulated by CT120, upon which CT120 might be involved in tumor invasiveness and metastasis.
In addition, CT120 might play an important role in tumor progression through modulating the expression of some candidate "lung tumor progression" genes including B-Raf, Rab-2, BAX, BAG-1, YB-1 and Cdc42.

Bibliography

Pubmed IDLast YearTitleAuthors
122701272002Molecular cloning and characterization of CT120, a novel membrane-associated gene involved in amino acid transport and glutathione metabolism.He X et al
156250162004Altered gene expression profiles of NIH3T3 cells regulated by human lung cancer associated gene CT120.He XH et al
200246282010Silencing of CT120 by antisense oligonucleotides could inhibit the lung cancer cells growth.Li Z et al
159273612006Down-regulation of CT120A by RNA interference suppresses lung cancer cells growth and sensitizes to ultraviolet-induced apoptosis.Pan D et al
161438122005Inhibitory effect of CT120B, an alternative splice variant of CT120A, on lung cancer cell growth.Pan DN et al

Citation

Zhiao Chen ; Xianghuo He

FAM57A (family with sequence similarity 57, member A)

Atlas Genet Cytogenet Oncol Haematol. 2010-08-01

Online version: http://atlasgeneticsoncology.org/gene/40183/fam57a