HSPH1 (heat shock 105kDa/110kDa protein 1)

2007-02-01   Takumi Hatayama , Nobuyuki Yamagishi 

Department of Biochemistry, Kyoto Pharmaceutical University, Misasagi, Yamashina, Kyoto 607-8414, Japan

Identity

HGNC
LOCATION
13q12.3
LOCUSID
ALIAS
HSP105,HSP105A,HSP105B,NY-CO-25
FUSION GENES

DNA/RNA

Atlas Image
Genomic organization of the mouse HSP105 gene. The linear map of the exon-intron structure is shown schematically. Exons are represented as numbered boxes. Two alternative splicing patterns gave rise to HSP105alpha and HSP105beta transcripts. ATG and TAG indicate the positions of initiation and termination codons, respectively. (DDBJ/EMBL/GenBank DNA databases with accession Nos. AB005267-AB005282).

Description

18 exons on 22 kb

Transcription

Hsp105alpha is transcribed constitutively and also by a variety of stresses. 4 kb mRNA Hsp105beta is an alternative spliced isoform only produced during heat shock at 42 degree.

Proteins

Atlas Image
Shematic structures of HSP105alpha and HSP105beta proteins. Shaded box represents the spliced out region of HSP105alpha which is lacking in HSP105beta.

Description

Hsp105alpha: 858 amino acids, 105 kDa; contains an ATP binding domain (residues 1-383), b-sheet domain (residues 384-511), loop domain (residues 512-607) and alpha-helix domain (residues 608-858). Hsp105beta: 814 amino acids, 90 kDa; contains an ATP binding domain (residues 1-383), b-sheet domain (residues 384-511), loop domain (residues 512-563) and alpha-helix domain (residues 564-814).

Expression

wide, highly expressed in brain

Localisation

Hsp105alpha, cytoplasmic; Hsp105beta, nuclear

Function

Hsp105alpha and Hsp105beta suppress the aggregation of denatured proteins; function as a substitute for Hsp70 family proteins to suppress the aggregation of denatured proteins in cells under severe stress; regulate substrate binding cycle of Hsp70/Hsc70 by inhibiting the ATPase activity of Hsp70/Hsc70.

Homology

With mouse apg-1, mouse apg-2, sea urchin egg receptor, C. elegans 86.9-kDa protein, A. thaliana hsp91 and S. cerevisiae SSE1, human hsp70 and human hsc70.

Implicated in

Entity name
Lung cancers
Prognosis
Poor
Oncogenesis
Low expression of hsp105 was identified as predictors of survival in lung adenocarcinomas.
Entity name
Colorectal cancers
Prognosis
Survival is not much more than 50% after 5 years.
Oncogenesis
Overexpression of hsp105 is a late event in the colorectal adenoma-carcinoma sequence.

Bibliography

Pubmed IDLast YearTitleAuthors
168278032006Synthetic small interfering RNA targeting heat shock protein 105 induces apoptosis of various cancer cells both in vitro and in vivo.Hosaka S et al
99314721999Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105.Ishihara K et al
145346952003Heat shock protein 105 is overexpressed in a variety of human tumors.Kai M et al
162322022005DNA vaccination of HSP105 leads to tumor rejection of colorectal cancer and melanoma in mice through activation of both CD4 T cells and CD8 T cells.Miyazaki M et al
169112852006Heat shock protein 105 is overexpressed in squamous cell carcinoma and extramammary Paget disease but not in basal cell carcinoma.Muchemwa FC et al
112377512001Gene cloning of immunogenic antigens overexpressed in pancreatic cancer.Nakatsura T et al
168571852006Hsp105 family proteins suppress staurosporine-induced apoptosis by inhibiting the translocation of Bax to mitochondria in HeLa cells.Yamagishi N et al
100664251999Genomic cloning and promoter analysis of the mouse 105-kDa heat shock protein (HSP105) gene.Yasuda K et al
85303611995Cloning and expression of murine high molecular mass heat shock proteins, HSP105.Yasuda K et al

Other Information

Locus ID:

NCBI: 10808
MIM: 610703
HGNC: 16969
Ensembl: ENSG00000120694

Variants:

dbSNP: 10808
ClinVar: 10808
TCGA: ENSG00000120694
COSMIC: HSPH1

RNA/Proteins

Gene IDTranscript IDUniprot
ENSG00000120694ENST00000320027Q92598
ENSG00000120694ENST00000320027A0A024RDS1
ENSG00000120694ENST00000380405Q92598
ENSG00000120694ENST00000380405A0A024RDQ0
ENSG00000120694ENST00000435381Q5TBM3
ENSG00000120694ENST00000445273A0A0A0MSM0
ENSG00000120694ENST00000602786R4GN69
ENSG00000120694ENST00000630972Q92598

Expression (GTEx)

0
50
100
150

Pathways

PathwaySourceExternal ID
Protein processing in endoplasmic reticulumKEGGko04141
Protein processing in endoplasmic reticulumKEGGhsa04141
Vesicle-mediated transportREACTOMER-HSA-5653656
Binding and Uptake of Ligands by Scavenger ReceptorsREACTOMER-HSA-2173782
Scavenging by Class F ReceptorsREACTOMER-HSA-3000484
Cellular responses to stressREACTOMER-HSA-2262752
Cellular response to heat stressREACTOMER-HSA-3371556
Regulation of HSF1-mediated heat shock responseREACTOMER-HSA-3371453

Protein levels (Protein atlas)

Not detected
Low
Medium
High

References

Pubmed IDYearTitleCitations
237375322013Hsp110 is a bona fide chaperone using ATP to unfold stable misfolded polypeptides and reciprocally collaborate with Hsp70 to solubilize protein aggregates.64
243188772014Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro.50
225057102012Human heat shock protein 105/110 kDa (Hsp105/110) regulates biogenesis and quality control of misfolded cystic fibrosis transmembrane conductance regulator at multiple levels.24
245129102014Patients with colorectal tumors with microsatellite instability and large deletions in HSP110 T17 have improved response to 5-fluorouracil–based chemotherapy.23
256459272015HSP105 recruits protein phosphatase 2A to dephosphorylate β-catenin.15
278196702017HSP110 promotes colorectal cancer growth through STAT3 activation.11
145346952003Heat shock protein 105 is overexpressed in a variety of human tumors.10
268317562016HSP110 T17 simplifies and improves the microsatellite instability testing in patients with colorectal cancer.10
180833462008HSP105 interacts with GRP78 and GSK3 and promotes ER stress-induced caspase-3 activation.9
218600232011Serological identification of HSP105 as a novel non-Hodgkin lymphoma therapeutic target.9

Citation

Takumi Hatayama ; Nobuyuki Yamagishi

HSPH1 (heat shock 105kDa/110kDa protein 1)

Atlas Genet Cytogenet Oncol Haematol. 2007-02-01

Online version: http://atlasgeneticsoncology.org/gene/40891/hsph1