MIR30A (microRNA 30a)

2012-07-01   Xiao-Bin Lv 

Dept of Breast Surgery, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, China

Identity

HGNC
MIR30A
LOCATION
6q13
LOCUSID
407029
ALIAS
MIRN30A,mir-30a

DNA/RNA

Atlas Image

Description

miR-30 microRNA precursor is a small non-coding RNA that regulates gene expression. Animal microRNAs are transcribed as ~70 nucleotide stem-loop precursor and subsequently processed by the Dicer enzyme to give a mature ~22 nucleotide product. In this case the mature sequence comes from both the 3 (miR-30) and 5 (mir-97-6) arms of the precursor. The products are thought to have regulatory roles through complementarity to mRNA. A screen of 17 miRNAs that have been predicted to regulate a number of breast cancer associated genes found variations in the microRNAs miR-17 and miR-30c-1, these patients were noncarriers of BRCA1 or BRCA2 mutations, lending the possibility that familial breast cancer may be caused by variation in these miRNAs. Members of the miR-30 family have been found to be highly expressed in heart cells.

Transcription

miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA.
Pre-miR Length: 71 bases.
gcgactgtaa acatcctcga ctggaagctg tgaagccaca gatgggcttt cagtcggatg tttgcagctg c

Pseudogene

No reported pseudogenes.

Proteins

Note

miRNAs are not translated into amino acids.

Implicated in

Entity name
Breast cancer
Disease
Overexpression of miR-30a suppressed the migration and invasiveness phenotypes of breast cancer cell lines. Moreover, reduced tumor expression of miR-30a in breast cancer patients was associated with an unfavorable outcome, including late tumor stage, lymph node metastasis, and worse progression (Cheng et al., 2012).
Prognosis
Higher expression levels of hsa-miR-30a-3p, hsa-miR-30c, and hsa-miR-182 were significantly associated with benefit of tamoxifen treatment and with longer PFS (Rodríguez-González et al., 2011).
Entity name
Colon carcinoma
Disease
miR-30a-5p was shown to be down-regulated in human colorectal cancer compared with normal colon mucosa. Overexpression of miR-30-5p suppresses proliferation colon cancer cell lines by targeting denticleless protein homolog (DTL) (Baraniskin et al., 2012).
Entity name
Non-small cell lung cancer
Disease
microRNA-30a expression was found inversely proportional to the invasive potential of various NSCLC cell lines, correlating positively with E-cadherin (epithelial marker) and negatively with N-cadherin (mesenchymal marker) expression. Lucerfise reporter assay indicates snail was a potential target of miR-30a (Kumarswamy et al., 2012).
Entity name
Squamous cell carcinoma
Note
Diagnosis: a 5-microRNA classifier (hsa-miR-210, hsa-miR-182, hsa-miR-486-5p, hsa-miR-30a, and hsa-miR-140-3p) that could distinguish SCC from normal lung tissues (Tan et al., 2011).
Entity name
Gastric cancer
Prognosis
Li et al identified seven-miRNA signature (miR-10b, miR-21, miR-223, miR-338, let-7a, miR-30a-5p, miR-126) for overall survival (p=0,0009) and relapse-free survival (p=0,0005) of gastric cancer patients (2010).
Entity name
Thyroid carcinomas
Disease
miR-30a-5p was down-regulated in Thyroid carcinomas in comparison to normal thyroid tissue (Visone, 2007).
Entity name
Chemotherapy resistance
Note
miR-30a in regulating beclin 1 expression and autophagy reveals a novel function for miRNA in a critical cellular event with significant impacts in cancer development, progression and treatment (Zhu et al., 2009).
miR-30a can sensitize tumor cells to cis-DDP via reducing beclin 1-mediated autophagy and that increasing miR-30a level in tumor cells represents a novel approach to enhance the efficacy of chemotherapy during cancer treatment (Zou et al., 2012).
Imatinib markedly inhibits expression of miR-30a in human CML cells. miR-30a is a potent inhibitor of autophagy by downregulating Beclin 1 and ATG5 expression. miR-30a mimic or knockdown of autophagy genes (ATGs) such as Beclin 1 and ATG5 by short hairpin RNA enhances imatinib-induced cytotoxicity and promotes mitochondria-dependent intrinsic apoptosis. In contrast, knockdown of miR-30a by antagomir-30a increases the expression of Beclin 1 and ATG5, and inhibits imatinib-induced cytotoxicity (Yu et al., 2012)

Bibliography

Pubmed IDLast YearTitleAuthors
224768512012MicroRNA-30a inhibits cell migration and invasion by downregulating vimentin expression and is a potential prognostic marker in breast cancer.Cheng CW et al
216339532012MicroRNA-30a inhibits epithelial-to-mesenchymal transition by targeting Snai1 and is downregulated in non-small cell lung cancer.Kumarswamy R et al
199519012010Survival prediction of gastric cancer by a seven-microRNA signature.Li X et al
204906522011MicroRNA-30c expression level is an independent predictor of clinical benefit of endocrine therapy in advanced estrogen receptor positive breast cancer.Rodríguez-González FG et al
218904512011A 5-microRNA signature for lung squamous cell carcinoma diagnosis and hsa-miR-31 for prognosis.Tan X et al
175637492007Specific microRNAs are downregulated in human thyroid anaplastic carcinomas.Visone R et al
223953612012Targeting microRNA-30a-mediated autophagy enhances imatinib activity against human chronic myeloid leukemia cells.Yu Y et al
195359192009Regulation of autophagy by a beclin 1-targeted microRNA, miR-30a, in cancer cells.Zhu H et al
221577652012MicroRNA-30a sensitizes tumor cells to cis-platinum via suppressing beclin 1-mediated autophagy.Zou Z et al

Other Information

Locus ID:

NCBI: 407029
MIM: 612329
HGNC: 31624
Ensembl: ENSG00000207827
miRBase:

Variants:

dbSNP: 407029
ClinVar: 407029
TCGA: ENSG00000207827
COSMIC: MIR30A

RNA/Proteins

Pathways

PathwaySourceExternal ID
MicroRNAs in cancerKEGGhsa05206
MicroRNAs in cancerKEGGko05206

References

Pubmed IDYearTitleCitations
195359192009Regulation of autophagy by a beclin 1-targeted microRNA, miR-30a, in cancer cells.151
216339532012MicroRNA-30a inhibits epithelial-to-mesenchymal transition by targeting Snai1 and is downregulated in non-small cell lung cancer.111
199654792009A high-resolution structure of the pre-microRNA nuclear export machinery.100
186326832008A set of differentially expressed miRNAs, including miR-30a-5p, act as post-transcriptional inhibitors of BDNF in prefrontal cortex.99
191389932008Genetic variations in microRNA-related genes are novel susceptibility loci for esophageal cancer risk.95
224768512012MicroRNA-30a inhibits cell migration and invasion by downregulating vimentin expression and is a potential prognostic marker in breast cancer.86
211874252011miR-29 and miR-30 regulate B-Myb expression during cellular senescence.82
222875602012MiR-30a-5p suppresses tumor growth in colon carcinoma by targeting DTL.73
245991342014miR-30-5p functions as a tumor suppressor and novel therapeutic tool by targeting the oncogenic Wnt/β-catenin/BCL9 pathway.67
238515092014MicroRNA-30a suppresses breast tumor growth and metastasis by targeting metadherin.64

Citation

Xiao-Bin Lv

MIR30A (microRNA 30a)

Atlas Genet Cytogenet Oncol Haematol. 2012-07-01

Online version: http://atlasgeneticsoncology.org/gene/51667/mir30a