RASL11B (RAS-like, family 11, member B)
2009-05-01 Stefan Lorkowski AffiliationInstitute of Nutrition, Friedrich Schiller University Jena, Dornburger Str. 25, 07743 Jena, Germany
DNA/RNA
Schematic representation of human RASL11B mRNAs and genomic organization of the human RASL11B gene. The human RASL11B gene consists of 4 exons encoding a transcript with a total length of 1962 bp. One shorter variant with a length of 766 bp was found. The ATG start and TGA stop codons are located in exons 1 and 4, respectively.
Description
Gene: 4508 bp
Chromosome: 4q12
mRNA: 1979 bp
Exon 1: 1-333
Exon 2: 334-390
Exon 3: 391-467
Exon 4: 468-1962
CDS: 192-936
Chromosome: 4q12
mRNA: 1979 bp
Exon 1: 1-333
Exon 2: 334-390
Exon 3: 391-467
Exon 4: 468-1962
CDS: 192-936
The human RASL11B gene spans about 4508 bp on chromosome 4q12 and comprises 4 exons encoding at least 2 different transcripts. Exons of the RASL11B gene are 333 bp (exon 1), 57 bp (exon 2), 77 bp (exon 3), and 1495 bp (exon 4) in size. Sizes of introns are 618 bp (intron 1), 1153 bp (intron 2), and 780 bp (exon 3). All splice sites have canonical boundaries, starting the intron with gt and ending with ag. A polyadenylation signal in the untranslated region of exon 4 is located at nucleotide position 1947.
Transcription
In addition to the full-length RASL11B transcript, a truncated polyadenylated transcript of 766 bp was reported.
Full-length transcript: 1962 bp mRNA, 744 bp open reading frame.
Truncated transcript: 766 bp mRNA, 574 bp open reading frame.
Full-length transcript: 1962 bp mRNA, 744 bp open reading frame.
Truncated transcript: 766 bp mRNA, 574 bp open reading frame.
Pseudogene
No pseudogenes reported.
Proteins
Domains within the human RASL11B protein. Domains positions are indicated with vertical purple lines and intron positions are indicated with vertical red lines both showing the exact position in the polypeptide sequence.
Description
RASL11B is a 248 amino acid protein containing a characteristic RAS GTPase domain with typical topology of a six-stranded beta-sheet surrounded by five alpha-helices. The RASL11B protein has no typical prenylation signal, indicating that it is probably not anchored to cellular membranes.
Expression
Expression of human RASL11B mRNA was investigated in 37 tissues and 5 cell types. In tissues, RASL11B transcript is widely expressed with highest levels in placenta. In cells RASL11B transcript shows highest abundance in primary macrophages.
Localisation
Cytosolic.
Function
Small GTPase belonging to a Ras subfamily of putative tumor suppressor genes.
Homology
The protein sequence of the RASL11 protein family is highly conserved within different species and contains five conserved regions motives that comprise the G-domain of small GTPases (P-loop, switch 1 and 2, G4 and G5 box).
Implicated in
Note
According to Stolle et al., RASL11B expression is induced during maturation of THP-1 monocytic cells into macrophages and in coronary artery smooth muscle cells after treatment with TGF-beta1 suggesting that RASL11B may play a role in developmental processes or in pathophysiologies such as inflammation or cancer.
Pezeron et al. demonstrated that Rasl11b modulates function of the EGF-CFC coreceptor one-eyed-pinhead (oep) in zebrafish independently of the TGFbeta/Nodal pathway, which is crucial for germ layer formation. Down regulation of Rasl11b partially rescued endodermal and prechordal plate defects of zygotic homozygous oep zebrafish mutants. Rasl11b inhibitory action was observed only in animals with oep-deficient backgrounds, suggesting that normal oep expression prevents function of Rasl11b. On the other hand, Rasl11b down regulation did not rescue mesendodermal defects in other Nodal pathway mutants.
Pezeron et al. demonstrated that Rasl11b modulates function of the EGF-CFC coreceptor one-eyed-pinhead (oep) in zebrafish independently of the TGFbeta/Nodal pathway, which is crucial for germ layer formation. Down regulation of Rasl11b partially rescued endodermal and prechordal plate defects of zygotic homozygous oep zebrafish mutants. Rasl11b inhibitory action was observed only in animals with oep-deficient backgrounds, suggesting that normal oep expression prevents function of Rasl11b. On the other hand, Rasl11b down regulation did not rescue mesendodermal defects in other Nodal pathway mutants.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 18197245 | 2008 | Rasl11b knock down in zebrafish suppresses one-eyed-pinhead mutant phenotype. | Pézeron G et al |
| 17628721 | 2007 | Cloning, genomic organization, and tissue-specific expression of the RASL11B gene. | Stolle K et al |
Other Information
Locus ID:
NCBI: 65997
MIM: 612404
HGNC: 23804
Ensembl: ENSG00000128045
Variants:
dbSNP: 65997
ClinVar: 65997
TCGA: ENSG00000128045
COSMIC: RASL11B
RNA/Proteins
| Gene ID | Transcript ID | Uniprot |
|---|---|---|
| ENSG00000128045 | ENST00000248706 | Q9BPW5 |
Expression (GTEx)
Protein levels (Protein atlas)
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 32878463 | 2020 | RASL11B gene enhances hyaluronic acid-mediated chondrogenic differentiation in human amniotic mesenchymal stem cells via the activation of Sox9/ERK/smad signals. | 5 |
| 32878463 | 2020 | RASL11B gene enhances hyaluronic acid-mediated chondrogenic differentiation in human amniotic mesenchymal stem cells via the activation of Sox9/ERK/smad signals. | 5 |
| 31432180 | 2019 | Upregulation of MAPK10, TUBB2B and RASL11B may contribute to the development of neuroblastoma. | 4 |
| 31432180 | 2019 | Upregulation of MAPK10, TUBB2B and RASL11B may contribute to the development of neuroblastoma. | 4 |
| 29968912 | 2018 | Study on the mechanism behind lncRNA MEG3 affecting clear cell renal cell carcinoma by regulating miR-7/RASL11B signaling. | 25 |
| 29968912 | 2018 | Study on the mechanism behind lncRNA MEG3 affecting clear cell renal cell carcinoma by regulating miR-7/RASL11B signaling. | 25 |
| 17628721 | 2007 | Cloning, genomic organization, and tissue-specific expression of the RASL11B gene. | 15 |
| 17628721 | 2007 | Cloning, genomic organization, and tissue-specific expression of the RASL11B gene. | 15 |
Citation
Stefan Lorkowski
RASL11B (RAS-like, family 11, member B)
Atlas Genet Cytogenet Oncol Haematol. 2009-05-01
Online version: http://atlasgeneticsoncology.org/gene/44265/rasl11b
