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Entity | Glioma cell and cell lines |
Note | In human glioma cell lines, knocking down of ZFX expression by lentivirus-mediated RNA interference (RNAi) shows that ZFX plays an important role in glioma cell proliferation, survival and growth potential in both subcutaneous and intracranial models in mice (Zhu et al., 2013). Furthermore, Zhou et al. detected ZFX expression in U251 cells using semi- quantitative PCR. They showed that expression of ZFX is significantly higher in glioma tissues compared to non glioma tissues using real-time RT-PCR. In the same vein, Knocking down of ZFX gene expression in U251 cells results in disturbed cell proliferation and increased apoptosis and arrested cells in S phase (Zhou et al., 2011). |
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Entity | Gastric cancer tissues and cells |
Note | ZFX gene expression was examined by quantitative real-time RT-PCR in 30 paired gastric tissue samples. Result shows that ZFX gene has differential expression in gastric tissues. There is a statistically significant association between the ZFX expression and different tumour types and grades (Nikpour et al., 2012). Furthermore, ZFX silencing inhibits gastric cancer cell growth in both in vitro and in vivo via modulating extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK-MAPK) pathway (Wu et al., 2013). |
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Entity | Non - small cell lung cancer (NSCLC) |
Note | ZFX overexpresses in NSCLC and its expression is correlated with lymph node metastasis. Knocking down of ZFX gene by short hairpin RNA interference (shRNA) in NSCLC cell line reduces cell viability and colony formation and causes cell cycle arrest (Jiang et al., 2012b). |
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Entity | Laryngeal squamous cell carcinoma (LSCC) |
Note | ZFX expression up-regulates in LSCC tissues compared to the non- tumoral tissues. Knock-down of ZFX by shRNA results in suppression of proliferation and colony-forming potential of infected Hep-2 human LSCC cells and induces enhancing of cell apoptosis. Furthermore, high expression of ZFX associates with LSCC progression and its decreased expression associates with declined tumor cell growth (Fang et al., 2012). |
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Entity | Osteosarcoma cells |
Note | Inhibition of ZFX expression by siRNA results in decreased proliferation, colony formation and invasion of Saos-2 cells. Silencing of ZFX gene expression causes cell cycle arrest in which greater portion of the cells existed in G1 phase and minor portion in S and G2/M phases (Jiang et al., 2013; Jiang et al., 2012a). |
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Entity | Prostate cancer |
Note | Prostate cancer tissues show higher expression of ZFX than that observed in normal adjacent tissues and BPH tissues. Furthermore, silencing the expression of ZFX in the prostate cancer cells effectively abolishes the cellular proliferation and colony-formation ability, and results in G1 phase cell cycle arrest. Moreover, inhibition of ZFX induces apoptosis by activating caspase-1, caspase-3 and caspase-9. (Jiang et al., 2012c). |
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Entity | Childhood B lineage acute lymphoblastic leukemia |
Note | ZFX protein expresses in human leukemia cell lines REH, HL-60, NB(4) and K562. Furthermore, ZFX mRNA expression increases in the newly-diagnosed ALL group while in the ALL complete remission group was significantly reduces. Moreover, ZFX mRNA level is significantly higher in Children with a poor prognosis than those with a good prognosis at diagnosis (Wang et al., 2013). |
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