| |  |
| |
| | Eight C2H2 zinc fingers and a proline-rich domain. Conserved linker sequence, TGEKP, reported to bind DNA with high affinity |
| |
| Description | Full-length ZNF217 cDNAs encode two open reading frames of 1,062 and 1,108 amino acids, due to alternative splicing of exon 4. Each predicted protein has eight C2H2 zinc fingers and a proline-rich domain. Sequence analysis of ZNF217 indicates a strong resemblance to members of the Kruppel-like family of zinc finger proteins. The eight zinc finger domains in ZNF217 are interspersed throughout the ZNF217 sequence and their pattern does not appear to fall into one of the three classes of C2H2 zinc finger proteins; triple-C2H2, multipleadjacent, and separated-paired fingers. The sixth and seventh zinc fingers in ZNF217 are separated by the conserved linker sequence, TGEKP, reported to bind DNA with high affinity. Database analysis indicates that this paired zinc finger region aligns with those in several members of the Delta-EF1/ZFH-1 family of two-handed zinc-finger homeodomain proteins, including Smad-Interacting Protein 1 (SIP-1). |
| Expression | ZNF217 is expressed at low levels in normal tissues. |
| Localisation | Nuclear |
| Function | ZNF217 protein localizes to the nucleus and co-immunoprecipitates with complexes containing the transcriptional corepressors CoREST and CtBP, histone deacetylases HDAC1 and HDAC2, and histone methyltransferases G9a and Eu-HMTase1. This strongly suggests that ZNF217 may function as part of a transcriptional repressor complex. |
| Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma. |
| Collins C, Rommens JM, Kowbel D, Godfrey T, Tanner M, Hwang SI, Polikoff D, Nonet G, Cochran J, Myambo K, Jay KE, Froula J, Cloutier T, Kuo WL, Yaswen P, Dairkee S, Giovanola J, Hutchinson GB, Isola J, Kallioniemi OP, Palazzolo M, Martin C, Ericsson C, Pinkel D, Albertson D, Li WB, Gray JW |
| Proceedings of the National Academy of Sciences of the United States of America. 1998 ; 95 (15) : 8703-8708. |
| PMID 9671742 |
| |
| The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells. |
| Nonet GH, Stampfer MR, Chin K, Gray JW, Collins CC, Yaswen P |
| Cancer research. 2001 ; 61 (4) : 1250-1254. |
| PMID 11245413 |
| |
| In situ analyses of genome instability in breast cancer. |
| Chin K, de Solorzano CO, Knowles D, Jones A, Chou W, Rodriguez EG, Kuo WL, Ljung BM, Chew K, Myambo K, Miranda M, Krig S, Garbe J, Stampfer M, Yaswen P, Gray JW, Lockett SJ |
| Nature genetics. 2004 ; 36 (9) : 984-988. |
| PMID 15300252 |
| |
| The candidate oncogene ZNF217 is frequently amplified in colon cancer. |
| Rooney PH, Boonsong A, McFadyen MC, McLeod HL, Cassidy J, Curran S, Murray GI |
| The Journal of pathology. 2004 ; 204 (3) : 282-288. |
| PMID 15476264 |
| |
| Detection of Her2/neu, c-MYC and ZNF217 gene amplification during breast cancer progression using fluorescence in situ hybridization. |
| Shimada M, Imura J, Kozaki T, Fujimori T, Asakawa S, Shimizu N, Kawaguchi R |
| Oncology reports. 2005 ; 13 (4) : 633-641. |
| PMID 15756435 |
| |
